PUBLICATIONS

SELECTED PUBLICATIONS

Chen W., Cao P., Liu Y., Yu A., Wang D., Chen L., Sundarraj R., Yuchi Z., Gong Y.*, Merzendorfer H.*, Yang Q.* (2022) Structural basis for directional chitin biosynthesis. Nature, doi: 10.1038/s41586-022-05244-5.

Duan Y., Wei Y., Xing M., Liu J., Jiang L., Lu Q., Liu X., Liu Y., Ang EL., Liao RZ., Yuchi Z.* , Zhao H.*, Zhang Y.*(2022) Anaerobic Hydroxproline Degradation Involving C-N Cleavage by a Glycyl Radical Enzyme. J Am Chem Soc, doi: 10.1021/jacs.2c01673.

Qin, J., Zhang, J., Lin, L., Haji-Ghassemi, O., Lin, Z., Woycechowsky, K.J., Van Petegem, F., Zhang, Y.*, Yuchi, Z.* (2022) Structures of PKA-phospholamban complexes reveal a mechanism of familial dilated cardiomyopathy. eLife, doi: 10.7554/eLife.75346.

Lu, Q.#, Wei, Y.#, Lin, L., Liu, J., Duan, Y., Li, Y., Zhai, W., Liu, Y., Ang, E., Zhao, H.*, Yuchi, Z.*, Zhang, Y.* (2021) The Glycyl Radical Enzyme Arylacetate Decarboxylase from Olsenella scatoligenes. ACS Catalysis, doi: 10.1021/acscatal.1c01253.

Ma, R.#, Haji-Ghassemi, O.#, Ma, D.#, Jiang, H.#, Lin, L., Yao, L., Samurkas, A., Li, Y., Wang, Y., Cao, P., Wu, S., Zhang, Y., Murayama, T., Moussian, B., Van Petegem, F.*,YuchiZ.* (2020) Structural Basis for Diamide Modulation of Ryanodine Receptor. Nature Chemical Biology, doi: 10.1038/s41589-020-0627-5.

Liu, J.#, Wei, Y., Lin, L., Teng, L., Yin, J., Lu, Q., Chen, J., Zheng, Y., Li, Y., Xu, R., Zhai, W., Liu, Y., Liu, Y., Cao, P., Ang, E., Zhao, H.*, Yuchi, Z.*,Zhang, Y.*(2020) Two radical-dependent mechanisms for anaerobic degradation of the globally abundant organosulfur compound dihydroxypropanesulfonateProc.Nati.Acad. Sci., doi: 10.1073/pnas.2003434117.

You, M.*, Ke,F.#, You,S.#, Wu, Z.#, Liu, Q.#, He, W.#, Baxter, S.#, Yuchi, Z.#, Vasseur, L.*, Gurr, G.*, Ward, C., Cerda, H., Yang, G., Peng, L., Jin, Y., Xie, M., Cai, L., Douglas, C., Isman, M., Goettel, M., Song, Q., Fan, Q., Wang-Pruski, G., Lees, D., Yue, Z.*, Bai, J., Liu, Y., Lin, L., Zheng, Y., Zeng, Z., Lin, S., Wang, Y., Zhao,Q., Xia, X., Chen, W., Chen, L., Zou, M., Liao, J., Gao, Q., Fang, X., Yin, Y., Yang, H., Wang, J., Han, L., Lin, Y., Lu, Y., and Zhuang, M. (2020) Variation among 532 genomes unveils the origin and evolutionary history of a global insect herbivore. Nature Communications, doi: 10.1038/s41467-020-16178-9.

Xu, T., and Yuchi, Z. (2019) Crystal structure of diamondback moth ryanodine receptor Repeat34 domain reveals insect-specific phosphorylation sites. BMC Biology, doi: 10.1186/s12915-019-0698-5.

Xing, M., Wei, Y., Zhou, Y., Zhang, J., Lin, L., Hu, Y., Hua, G., Nanja, A., Liu, D., Wang, F., Guo, C., Tong, Y., Li, M., Liu, Y., Ang, EL., Zhao, H.*, Yuchi, Z.*, and Zhang, Y.* (2019) Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria. Nature Communications, doi: 10.1038/s41467-019-09618-8.

Yuchi, Z., Wong King Yuen, S.M., Lau, K., Underhill, A., Cornea, R., Fessenden,J.D., and Van Petegem, F. (2015) Crystal structures of Ryanodine Receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant. Nature Communications, 6:7947 doi: 10.1038/ncomms8947.

2022

54.Lianyun Lin.; Heng J.; Hadiatullah, H.; Ruifang, Ma.; Henryk, K.; Yucheng, G.; Yuchi, Z.* Calmodulin Modulation of Insect Ryanodine Receptors. Journal of Agricultural and Food Chemistry. doi: 10.1021/acs.jafc.2c07519

53.Ye, M.; Xiong, L.; Dong, Y.; Xie, C.; Zhang, Z.; Shen, L.; Li, Z.; Yue, Z.; Jiang, P.; Yuchi, Z.; You, M.*, Merzendorfer H.*; You, S.*, Merzendorfer H.*The Potential Role of the Methionine Aminopeptidase Gene PxMetAP1 in a Cosmopolitan Pest for Bacillus thuringiensis Toxin Tolerance. Int. J. Mol. Sci. 202223, 13005. https://doi.org/10.3390/ijms232113005

52. Chen W., Cao P., Liu Y., Yu A., Wang D., Chen L., Sundarraj R., Yuchi Z., Gong Y.*, Merzendorfer H.*, Yang Q.*Structural basis for directional chitin biosynthesis. Nature, doi: 10.1038/s41586-022-05244-5.

51. Guo X., Wang R., Ma R., Fan X., Gao Y., Zhang X., Yuchi Z.*, Wu HY.* Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli. Protein Expr Purif, doi: 10.1016/j.pep.2022.106112.

50. Duan Y., Wei Y., Xing M., Liu J., Jiang L., Lu Q., Liu X., Liu Y., Ang EL., Liao RZ., Yuchi Z.* , Zhao H.*, Zhang Y.* Anaerobic Hydroxproline Degradation Involving C-N Cleavage by a Glycyl Radical Enzyme. J Am Chem Soc, doi: 10.1021/jacs.2c01673.

49. Qin, J., Zhang, J., Lin, L., Haji-Ghassemi, O., Lin, Z., Woycechowsky, K.J., Van Petegem, F., Zhang, Y.*, Yuchi, Z.* Structures of PKA-phospholamban complexes reveal a mechanism of familial dilated cardiomyopathy. eLife, doi: 10.7554/eLife.75346.

48. Yang, Q., Yuchi, Z. Ryanodine Receptors: Current Progress and Future Perspective. Curr Pharm Des, doi: 10.2174/138161282801211223123314.

47. Li, Y., Zhang, Y., Feng, S., Dai, P., Liu, F., Shang, Y., Qin, J., Yuchi, Z., Wang, Z., Zhao, Y. Tailored Trojan horse nanocarriers for enhanced redox-responsive drug delivery. J Control Release, doi: 10.1016/j.jconrel.2022.01.006.

46. Hadiatullah, H., Zhang, Y., Samurkas, A., Xie, Y., Sundarraj, R., Zuilhof, H., Qiao, J.*, Yuchi, Z.* Recent progress in the structural study of ion channels as insecticide targets. Insect Science, doi: 10.1111/1744-7917.13032.

45. Zhang, Z., Sun, P., Zhao, J., Zhang, H., Wang, X., Li, L., Xiong, L., Yang, N., Li, Y.*, Yuchi, Z.*, Li, Z.* Design, synthesis and biological activity of diamide compounds based on 3-substituent of the pyrazole ring. Pest Management Science, doi: 10.1002/ps.6826.

44. Ye, M., Nayak, B., Xiong, L., Xie, C., Dong, Y., You, M., Yuchi, Z.*, You, S.* The Role of Insect Cytochrome P450s in Mediating Insecticide Resistance. Agriculture, doi: 10.3390/agriculture12010053. 

2021

43. Jia, Z., Liu, C., Chen, Y., Jiang, H., Wang, Z., Yao, J., Yang, J., Zhu, J., Zhang, B., Yuchi, Z. Crystal structures of the SARS-CoV-2 nucleocapsid protein C-terminal domain and development of nucleocapsid-targeting nanobodies. FEBS J, doi: 10.1111/febs.16239. 

42. Shao, J., Zhang, Q., Wei, J., Yuchi, Z., Cao, P., Li, S., Wang, S., Xu, J., Yang, S., Zhang, Y., Wei, J., Tian, J. Synthesis, crystal structures, anticancer activities and molecular docking studies of novel thiazolidinone Cu(II) and Fe(III) complexes targeting lysosomes: special emphasis on their binding to DNA/BSA. Dalton Trans, doi: 10.1039/d1dt02180j. 

41. Liu, F., Zhu, J., Dai, P., Deng, J., Qin, J., Yuchi, Z., Fan, A., Wang, Z., Zhao, Y. Double-Lock Nanomedicines Enable Tumor-Microenvironment-Responsive Selective Antitumor Therapy.  Advanced Functional Materials, doi: 10.1002/adfm.202009157. 

40. Samurkas, A., Yao, L., Hadiatullah, H., Ma, R., Xie, Y., Sundarraj, R., Zuilhof, H., Yuchi, Z. Ryanodine receptor as insecticide target. Curr Pharm Des, doi: 10.2174/1381612827666210902150224. 

39. Liu, S., Yang, L., Kong, F., Zhao, J., Yao, L., Yuchi, Z., Ma, Q., Xie, Q., Zhou, L., Guo, M., Dai, H., Zhao, Y., Luo, D. Three New Quinazoline-Containing Indole Alkaloids From the Marine-Derived Fungus Aspergillus sp. HNMF114. Front Microbiol, doi: 10.3389/fmicb.2021.680879.

38. Li, F.#, Lin, L.#, Wang, H., Duan, W., Yuan, L., Cao, P., Yuchi, Z.*, Wu, S.* Functional characterization of knockdown resistance mutations in the plant bug, Apolygus lucorum Meyer-Dür. Pestic Biochem Physiol, doi: 10.1016/j.pestbp.2021.104874.

37. Lu, Q.#, Wei, Y.#, Lin, L., Liu, J., Duan, Y., Li, Y., Zhai, W., Liu, Y., Ang, E., Zhao, H.*, Yuchi, Z.*, Zhang, Y.* The Glycyl Radical Enzyme Arylacetate Decarboxylase from Olsenella scatoligenes. ACS Catalysis, doi: 10.1021/acscatal.1c01253.

36. Touat-Hamici, Z.#, Blancard, M.#, Ma, R.#, Lin, L., Iddir, Y., Denjoy, I., Leenhardt, A., Yuchi, Z.*, Guicheney, P.* A SPRY1 domain cardiac ryanodine receptor variant associated with short-coupled torsade de pointes. Scientific Reports, doi: 10.1038/s41598-021-84373-9.

 

2020

35. Wang, R.#, Lin, L.#, Zheng, Y.#, Cao, P., Yuchi, Z.*, Wu, H.* Identification of 2-PMPA as a novel inhibitor of cytosolic carboxypeptidases. Biochemical and Biophysical Research Communications, doi: 10.1016/j.bbrc.2020.10.029.

34. Ma, R.#, Haji-Ghassemi, O.#, Ma, D.#, Jiang, H.#, Lin, L., Yao, L., Samurkas, A., Li, Y., Wang, Y., Cao, P., Wu, S., Zhang, Y., Murayama, T., Moussian, B., Van Petegem, F.*,YuchiZ.* Structural Basis for Diamide Modulation of Ryanodine Receptor. Nature Chemical Biology, doi: 10.1038/s41589-020-0627-5.

33. Zhou, Y.#, Wang, W.#, Salauddin, N., You, M.,You, S.*,YuchiZ.* Crystal structure of the N-terminal domain of ryanodine receptor from the honeybee, Apis mellifera. Insect Biochemistry and Molecular Biology, doi: 10.1016/j.ibmb.2020.103454.

32. Tikhonov, D.*, Lin, L., Yang, D., Yuchi, Z.*, Zhorov, B.* Phenylalkylamines in Calcium Channels: Computational Analysis of Experimental Structures. Journal of Computer-Aided Molecular Design, doi: 10.1007/s10822-020-00330-0.

31. Liu, J.#, Wei, Y., Lin, L., Teng, L., Yin, J., Lu, Q., Chen, J., Zheng, Y., Li, Y., Xu, R., Zhai, W., Liu, Y., Liu, Y., Cao, P., Ang, E., Zhao, H.*, Yuchi, Z.*,Zhang, Y.* Two radical-dependent mechanisms for anaerobic degradation of the globally abundant organosulfur compound dihydroxypropanesulfonate. Proc.Nati.Acad. Sci., doi: 10.1073/pnas.2003434117.

30. Wang, L., Lin, L., Duan, W., Wang, H., Li, F., Zhang, K., Cao, P., Yuchi, Z.*, Wu, S.* Two classic mutations in the linker-helix IIL45 and segment IIS6 of Apolygus lucorum sodium channel confer pyrethroid resistance. Pest Management Science, doi: 10.1002/ps.5944.

29. You, M.*, Ke,F.#, You,S.#, Wu, Z.#, Liu, Q.#, He, W.#, Baxter, S.#, Yuchi, Z.#, Vasseur, L.*, Gurr, G.*, Ward, C., Cerda, H., Yang, G., Peng, L., Jin, Y., Xie, M., Cai, L., Douglas, C., Isman, M., Goettel, M., Song, Q., Fan, Q., Wang-Pruski, G., Lees, D., Yue, Z.*, Bai, J., Liu, Y., Lin, L., Zheng, Y., Zeng, Z., Lin, S., Wang, Y., Zhao,Q., Xia, X., Chen, W., Chen, L., Zou, M., Liao, J., Gao, Q., Fang, X., Yin, Y., Yang, H., Wang, J., Han, L., Lin, Y., Lu, Y., and Zhuang, M. Variation among 532 genomes unveils the origin and evolutionary history of a global insect herbivore. Nature Communications, doi: 10.1038/s41467-020-16178-9.

28. Samurkas, A., Fan, X., Ma, D., Sundarraj, R., Lin, L., Yao, L., Ma, R., Jiang, H., Cao, P., Gao, Q.,  and Yuchi, Z. Discovery of potential species-specific green insecticides targeting lepidopteran ryanodine receptor. Journal of Agricultural and Food Chemistry, doi: 10.1021/acs.jafc.0c01063.

27. Li, Z.#, Jiang, H.# ,Liu, S., Li, Y.*, Yuchi, Z.*, Gao, Q.* Ryanodine receptor-targeting small molecule fluorescent probes enables non-isotopic labeling and efficient drug screening for green insecticides. Analytica Chimica Acta, doi: 10.1016/j.aca.2020.02.047.

26. Zhou, Y., Ma, D., Lin, L., You, M., Yuchi, Z.*,  and You S.* Crystal structure of ryanodine receptor SPRY2 domain from the diamondback moth provides insights for development of novel insecticides. Journal of Agricultural and Food Chemistry, doi: 10.1021/acs.jafc.9b08151.

2019

25. Lin, L., Hao, Z., Cao, P, and Yuchi, Z. Homology modeling and docking study of diamondback moth ryanodine receptor reveals the mechanisms for channel activation, insecticide binding and resistance. Pest Management Science, doi: 10.1002/ps.5640.

24. Xu, T., and Yuchi, Z. Crystal structure of diamondback moth ryanodine receptor Repeat34 domain reveals insect-specific phosphorylation sites. BMC Biology, doi: 10.1186/s12915-019-0698-5.

23. Li, M., Wei, Y., Yin, J., Lin, L., Zhou, Y., Hua, G., Cao, P., Ang, E., Zhao, H.*, Yuchi, Z.*, Zhang, Y.* Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense. Biochemical Journal, doi: 10.1042/BCJ20190206.

22. Zhou, Y., Wei, Y., Urs, A. N. N., Lin, L., Xu, T., Hu, Y., Ang EL., Zhao H.*, Yuchi Z.*, and Zhang, Y.* Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense. Bioscience Reports,  doi: 10.1042/BSR20190715.

21. Haji-Ghassemi, O., Yuchi, Z., and Van Petegem, F. The Cardiac Ryanodine Receptor Phosphorylation Hot Spot Embraces PKA in a Phosphorylation-Dependent Manner. Molecular Cell, doi: 10.1016/j.molcel.2019.04.019.

20. Xing, M., Wei, Y., Zhou, Y., Zhang, J., Lin, L., Hu, Y., Hua, G., Nanja, A., Liu, D., Wang, F., Guo, C., Tong, Y., Li, M., Liu, Y., Ang, EL., Zhao, H.*, Yuchi, Z.*, and Zhang, Y.* Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria. Nature Communications, doi: 10.1038/s41467-019-09618-8.

19. Alvarado, FJ., Bos, JM., Yuchi, Z., Valdivia, CR., Hernandez, JJ., Zhao, YT., Henderlong, DS., Chen, Y., Booher, TR., Marcou, CA., Van Petegem, F., Ackerman, MJ., and Valdivia, HH. Cardiac hypertrophy and arrhythmia in mice induced by a mutation in ryanodine receptor 2. JCI Insight, doi: 10.1172/jci.insight.126544.

18. Zhou, Y., Wei, Y., Lin, L., Xu, T., Ang, EL., Zhao, H., Yuchi, Z.*, Zhang, Y*. Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca. Biochem J, doi: 10.1042/BCJ20190005.

17. Zhang, J., Nanjaraj Urs, A N., Lin, L., Zhou, Y., Hu, Y., Hua, G., Gao, Q., Yuchi, Z.*, and Zhang, Y.* Structure of glycerol dehydrogenase (GldA) from Escherichia coli. Acta Crystallographica Section F: Structural Biology Communications, doi:
10.1107/S2053230X19000037.

2018

16. Nanjaraj Urs, A N., Hu, Y., Li, P., Yuchi, Z., Chen, Y., Zhang, Y. Cloning and expression of a nonribosomal peptide synthetase to generate blue rose. ACS Synth Biol, doi: 10.1021/acssynbio.8b00187.

15. Nayak, B. C., Wang, J., Lin, L., He, W., You,  M., * Yuchi, Z. * Crystal   Structure of the N-Terminal Domain of the Ryanodine Receptor from Plutella   Xylostella. JoVE, doi: 10.3791/58568.

14. Lin, L., Liu, C., Nayak, BC., He, W., You, M., *and Yuchi, Z. * A two-step purification strategy using calmodulin as an affinity tag. J Chromatogr A1544, 16-22, doi: 10.1016/j.chroma.2018.02.045.

13. Lin, L., Liu, C., Qin, J., Wang, J., Dong, S., Chen, W., He, W., Gao, Q., You, M., *Yuchi, Z. * Crystal structure of ryanodine receptor N-terminal domain from Plutella xylostella reveals two potential species-specific insecticide-targeting sites. Insect Biochemistry and Molecular Biology, doi: 10.1016/j.ibmb.2017.11.009.

12. Xiong, J., Liu, X., Gong, Y., Zhang, P., Qiang, S., Zhao, Q., Guo, R., Qian,Y., Wang, L., Zhu, L., Wang, R., Hao, Z., Wen, H., Zhang, J., Tang, K., Zang, WF., Yuchi, Z., Chen, H., Chen, SRW., Zheng, W., Wang, SQ., Xu, YW., Liu, Z. Pathogenic mechanism of a catecholaminergic polymorphic ventricular tachycardia causing-mutation in cardiac calcium release channel RyR2. J Mol Cell Cardiol, doi: 10.1016/j.yjmcc.2018.02.014.

2017

11. Roston, TM., Yuchi, Z., Kannankeril, PJ., Hathaway, J., Vinocur, JM., Etheridge, SP., Potts, JE., Maginot, KR., Salerno, JC., Cohen, MI., Hamilton, RM., Pflaumer, A., Mohammed, S., Kimlicka, L., Kanter, RJ., LaPage, MJ., Collins, KK., Gebauer, RA., Temple, JD., Batra, AS., Erickson, C., Miszczak-Knecht ,M., Kubuš, P., Bar-Cohen, Y., Kantoch, M., Thomas, VC., Hessling, G., Anderson, C., Young, ML., Choi, SH., Cabrera Ortega, M., Lau, YR., Johnsrude, CL., Fournier, A., Van Petegem, F., Sanatani, S. The clinical and genetic spectrum of catecholaminergic polymorphic ventricular tachycardia: findings from an international multicentre registry. Europace, doi: 10.1093/europace/euw389.

2016

10. Yuchi, Z., and Van Petegem, F. Ryanodine Receptors under the MagnifyingLens: insights and limitations from current cryo-electron microscopy and X-ray crystallography studies. Cell Calcium, doi: 10.1016/j.ceca.2016.04.003.

2015

9. Yuchi, Z., Wong King Yuen, S.M., Lau, K., Underhill, A., Cornea, R., Fessenden,J.D., and Van Petegem, F. Crystal structures of Ryanodine Receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant. Nature Communications, doi: 10.1038/ncomms8947.

8. Wang, L., Meng, X., Yuchi, Z., Zhao, Z., Xu, D., Fedida, D., Wang, Z., and Huang, C. De Novo mutation in the SCN5A gene associated with Brugada syndrome. Cellular Physiology and Biochemistry, doi: 10.1159/000430189.

2013

7. Kimlicka, L., Tung, C. C., Carlsson, A. C., Lobo, P. A., Yuchi, Z., and Van Petegem, F. The cardiac ryanodine receptor N-terminal region contains an anion binding site that is targeted by disease mutations. Structuredoi: 10.1016/j.str.2013.06.012.

2012

6. Yuchi, Z., Lau, K., and Van Petegem, F. Disease mutations in the Ryanodine Receptor Central Region: Crystal structures of a phosphorylation hot spot domain. Structure, doi: 10.1016/j.str.2012.04.015.

2011

5. Yuchi, Z., and Van Petegem, F. Common allosteric mechanisms between ryanodine and inositol-1,4,5-trisphosphate receptors. Channelsdoi: 10.4161/chan.5.2.14313.

2009

4. Yuchi, Z., Pau, V. P., Lu, B.X., Junop, M., and Yang, D.S. An engineered right-handed coiled coil domain imparts extreme thermostability to the KcsA channel. FEBS J, doi:
10.1111/j.1742-4658.2009.07327.x.

2008

3. Yuchi, Z., Pau, V. P., and Yang, D. S.GCN4 enhances the stability of the pore domain of potassium channel KcsA. FEBS J, doi: 10.1111/j.1742-4658.2008.06747.x.

2007

2. Pau, V. P., Zhu, Y., Yuchi, Z., Hoang, Q. Q., and Yang, D. S. Characterization of the C-terminal domain of a potassium channel from Streptomyces lividans (KcsA). J Biol Chem, doi: 10.1074/jbc.M703277200

2004

1. Hu, J. H., Zhang, J. F., Ma, Y. H., Jiang, J., Yang, N., Li, X. B., Yu Chi, Z. G., Fei, J., and Guo, L. H. Impaired reproduction in transgenic mice overexpressing Gamma-aminobutyric acid transporter I (GAT1). Cell Researchdoi: 10.1038/sj.cr.7290202.

 

BOOK CHAPTERS

2. Nayak, BC., and Yuchi, Z. (2018) Calmodulin-based recombinant protein purification strategies. Calmodulin: Structure, Mechanisms and Functions, Nova Science Publishers

1. Yuchi, Z., Kimlicka, L., and Van Petegem, F. (2013) Structural insights into disease mutations of the Ryanodine Receptor. Genetic Disorders, Intech

 

PATENTS

4. Yuchi, Z and Yao, L. (2021) Tools and methods for screening insecticides. (Application number: 202110884938.4)

3. Yuchi, Z., Ma, R., Ma, D., Jiang, H., Zhang, J., Li, Z., Li, Y., Zhou, S., Mao, M., Van Petegem, F., Haji-Ghassemi, O., Murayama, T. (2021) The new uses of compounds. (Application number: 202110771594.6)

2. Ji, J., An, T., Si, Z., and Yuchi, Z. (2017) A highly effective method for tissue culture of lily flowers. (Application number: 201710631938.7)

1. Yuchi, Z., Lin, L., and Chen, L. (2018) A novel purification protocol using camodulin. (Application number: 201810033972.9)